A Secret Weapon For flow cytometers

Facts-enriched characterization of cell populations. Morphological data from photographs can incorporate to the richness of flow cytometry details. One example is, the determine shows an in any other case typical apoptosis assay using Annexin V and PI, adding cell imaging to characterize cells in Each individual inhabitants to reveal morphologically distinctive characteristics. These insights could not are already attained from multiplex staining on your own.

Regardless of whether you’re a novice or a specialist, discover our resources to progress your research and expertise in flow cytometry.

(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The amount of singlets accessible for Evaluation raises once the user trains and employs a far more correct product.

The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating by using a superior-pace brightfield digicam for simultaneous substantial throughput flow cytometry and higher resolution brightfield imaging.

"A picture is not pretty really worth a thousand dots, nevertheless it definitely really helps to see photos. It is excellent to see photographs that correlate with staining. For example, AnnexinV-optimistic cells that happen to be morphologically different than healthier cells, or CD14+ monocytes that happen to be bigger and more textured than CD3+/CD4+/CD14- lymphocytes.

Here, an experienced user has gated singlets confidently. Right after analyzing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of greater than four% aggregates.

Optimizing gating technique. Even robust guide singlet gating is error-vulnerable and remains a subjective choice issue in Just about all flow cytometry assays. Imaging can be employed to substantiate and regulate gates to incorporate only solitary cells of interest.

Our researchers will style and design panels for you personally as again and again you need—free of charge, no solution purchase required.

Early signs of this contamination had to begin with been dismissed as particles. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also permits you to use morphological characteristics to discover appealing subpopulations that will not be obvious from flow cytometry facts by itself.

Buyers can view event visuals in authentic-time to verify sample high-quality, improve protocols, and document the morphology of every cell population discovered for analysis. Imaging seize also Advantages from acoustic concentrating, which helps to situation the cells so that a pointy, centered image is received.

AI-driven automated impression Evaluation with the Attune Cytometric Program interprets event characteristics into distinctive morphology parameters that can be coupled with conventional fluorescence and scatter parameters to reveal extremely reproducible population modifications and distinctive subsets.

Application note: Automatic picture analysis decreases user-to-consumer variability in flow cytometry gating strategies

Leverage the power of Superior technology and innovation to speed up your discoveries. Belief from the Attune Flow Cytometer relatives to provide the reliability, pace, and precision that set your perform apart.

No wash/no lyse leukocyte analysis with imaging. Cells were being acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Pro UX/UI allows customers to practice AI versions for expanded programs. (A) The “Educate Design” characteristic gives annotation resources and genuine-time model training precision.

At the center of each and every Attune Flow Cytometer lies our innovative acoustic concentrating technologies, which makes use of audio waves to meticulously align cells throughout the flow cell. This ground breaking strategy surpasses standard hydrodynamic strategies, leading to more rapidly processing speeds, Excellent details excellent, and greater sample throughput.

The Attune Xenith cytometer can be a superior-parameter spectral here analyzer, providing equally spectral unmixing and standard flow abilities for extensive and flexible cell Investigation.

Agilent Benefit Promise guarantees you at the very least a decade of instrument use through the date you purchase. Usually, We're going to credit score you Along with the residual value of the system toward an upgraded design.

In this article, we demonstrate that by gating techniques applying impression-based mostly quantitative parameters can distinguish interacting cells from coincident functions to additional properly assess interacting cells.